A Computational Model of Light-Sheet Fluorescence Microscopy using Physically-based Rendering
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Date
2015
Journal Title
Journal ISSN
Volume Title
Publisher
The Eurographics Association
Abstract
We present a physically-based computational model of the light sheet fluorescence microscope (LSFM). Based on Monte Carlo ray tracing and geometric optics, our method simulates the operational aspects and image formation process of the LSFM. An extension for previous fluorescence models is developed to account for the intrinsic characteristics of fluorescent dyes in order to accurately simulate light interaction with fluorescent-tagged biological specimen. This extension was quantitatively validated against the fluorescence brightness equation and experimental spectra of different dyes. We demonstrate first results of our rendering pipeline to a simplified brain tissue model reconstructed from the somatosensory cortex of a young rat.
Description
@inproceedings{10.2312:egp.20151038,
booktitle = {EG 2015 - Posters},
editor = {B. Solenthaler and E. Puppo},
title = {{A Computational Model of Light-Sheet Fluorescence Microscopy using Physically-based Rendering}},
author = {Abdellah, Marwan and Bilgili, Ahmet and Eilemann, Stefan and Markram, Henry and Schürmann, Felix},
year = {2015},
publisher = {The Eurographics Association},
ISBN = {},
DOI = {10.2312/egp.20151038}
}